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| Registros recuperados: 54 | |
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| Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle. |
| Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect... |
| Tipo: Text |
Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf |
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| Nascimento,C.C.F.; Padula,N.; Milani,J.G.P.O.; Shimano,A.C.; Martinez,E.Z.; Mattiello-Sverzut,A.C.. |
| The objective of the present study was to determine to what extent, if any, swimming training applied before immobilization in a cast interferes with the rehabilitation process in rat muscles. Female Wistar rats, mean weight 260.52 ± 16.26 g, were divided into 4 groups of 6 rats each: control, 6 weeks under baseline conditions; trained, swimming training for 6 weeks; trained-immobilized, swimming training for 6 weeks and then immobilized for 1 week; trained-immobilized-rehabilitated, swimming training for 6 weeks, immobilized for 1 week and then remobilized with swimming for 2 weeks. The animals were then sacrificed and the soleus and tibialis anterior muscles were dissected, frozen in liquid nitrogen and processed histochemically (H&E and mATPase).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swimming training; Female rat skeletal muscles; Immobilization; Morphometry; ATPase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900013 |
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| Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep. |
| A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60ºC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,... |
| Tipo: Journal article |
Palavras-chave: Esterification; Immobilization; Lipase; Thermostability. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011 |
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| Cação-Benedini,L.O.; Ribeiro,P.G.; Prado,C.M.; Chesca,D.L.; Mattiello-Sverzut,A.C.. |
| Extracellular matrix and costamere proteins transmit the concentric, isometric, and eccentric forces produced by active muscle contraction. The expression of these proteins after application of passive tension stimuli to muscle remains unknown. This study investigated the expression of laminin and dystrophin in the soleus muscle of rats immobilized with the right ankle in plantar flexion for 10 days and subsequent remobilization, either by isolated free movement in a cage or associated with passive stretching for up to 10 days. The intensity of the macrophage response was also evaluated. One hundred and twenty-eight female Wistar rats were divided into 8 groups: free for 10 days; immobilized for 10 days; immobilized/free for 1, 3, or 10 days; or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Muscle stretching; Soleus muscle; Laminin; Dystrophin; Mechanotransduction. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600483 |
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| Sugahara,Vanessa Hitomi; Varéa,Geni da Silva. |
| Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842 |
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| Alves,J.S.M.; Leal-Cardoso,J.H.; Santos-Júnior,F.F.U.; Carlos,P.S.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Ceccatto,V.M.; Barbosa,R.. |
| Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Sciatic nerve; Compound action potential; Conduction velocity; Rheobase; Chronaxy. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715 |
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| Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Bosi,Paula Lima; Delfino,Gabriel Borges; Montebelo,Maria Imaculada de Lima; Guirro,Rinaldo Roberto de Jesus; Silva,Carlos Alberto da; Polacow,Maria Luiza Ozores. |
| The aim of this work was to investigate the effect of the neuromuscular electrical stimulation (ES) on the metabolic and morfometric profile of the tibialis anterior muscle, antagonist to the soleus muscle which was stimulate, under the joint immobilization condition of the ankle for 7 and 15 days. The immobilization promoted the reduction in the muscle mass (I7: 17.36%, I15: 20.83%), in the glycogen content (I7: 48%, I15: 48%), in the muscle fibers areas (I7: 27%, I15: 40%) and increase in intramuscular connective tissue density (I7: 122%, I15: 206%). The EE didn't promote significant alterations in the mass of the immobilized groups, however, promoted increase in the glycogen (IEE7: 31.25%; IEE15: 56.25%), reduction in the muscle fibers areas (IEE7: 14%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Muscle disuse; Metabolic; Morphometric; Neuromuscular electrical stimulation; Rehabilitation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100011 |
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| Dzionek,Anna; Wojcieszyńska,Danuta; Guzik,Urszula. |
| Bioremediation of contaminated groundwater or soil is currently the cheapest and the least harmful method of removing xenobiotics from the environment. Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes, reduces their costs, and also allows for the multiple use of biocatalysts. Among the developed methods of immobilization, adsorption on the surface is the most common method in bioremediation, due to the simplicity of the procedure and its non-toxicity. The choice of carrier is an essential element for successful bioremediation. It is also important to consider the type of process ( n s tu or ex s tu), type of pollution, and properties of immobilized microorganisms. For these reasons,... |
| Tipo: Journal article |
Palavras-chave: Adsorption; Bioethanol production; Contamination; Heavy metals; Immobilization; Immobilized microorganisms; Natural carriers; Wine alterations; Wood barrels; Xenobiotics. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500008 |
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| Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong. |
| Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and κ-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized κ-carrageenase were investigated. Results The κ-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for κ-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U κ-carrageenase for 20 mg of magnetic nanoparticles, a 2-h... |
| Tipo: Journal article |
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001 |
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| da Silva,C.A.; Guirro,R.R.J.; Polacow,M.L.O.; Cancelliero,K.M.; Durigan,J.L.Q.. |
| The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6): control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed: glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Hypotrophy; Joint; Orthoses; Physical therapy. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700016 |
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| Registros recuperados: 54 | |
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